Heterokaryosis and you may parasexual recombination within the pathogenic strains out of Fusarium oxysponrm
V. Heterokaryosis and you may parasexuality
Utilize the “0”spot for one of the biological parents and you can note the strain amount to the plate. Make use of the layout on the replicator. Incubate 2-three days. Replicate the segregants to the a number of decide to try plates having fun with a replicator that have, elizabeth.grams., 21 needles. Draw the latest dishes having a variety. Incubate 2-three days. Score the test dishes and you can record the brand new phenotypes on the rating table. Just be sure to influence the fresh ploidy of the territories with the foundation from the newest indicators. Take a look at ploidy out of not sure colonies. Make a list of the fresh genotypes (you can utilize a utility). Influence the fresh portion of the latest recombinants on various other markers. And therefore indicators try connected? Are you willing to discover intrachromosomal recombination?Read More »Heterokaryosis and you may parasexual recombination within the pathogenic strains out of Fusarium oxysponrm